| Reagent | Final concentration | Volume |
|---|---|---|
| Primer A (10 μM) | 1 μM | 1 μL |
| Primer B or Primer C (10 μM) | 1 μM | 1 μL |
| Template (genome) | N/A | 1 μL |
| GoTaq Green Master Mix 2× (Promega) | 1× | 5 μL |
| Nuclease free water | N/A | 2 μL |
| Total | N/A | 10 μL |
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