Chemicals, peptides, and recombinant proteins |
|
Dithiothreitol (DTT) |
Oakwood Chemical |
3483123 |
Fetal bovine serum |
Sigma |
F4135 |
SNAP-Cell 647-SiR |
New England Biolabs |
S9102S |
CLIP-Cell 505 |
New England Biolabs |
S9217S |
MG-132 proteosome inhibitor |
Cayman Chemical |
10012628 |
Magnetic Myc resin |
Thermo Fisher Scientific |
88842 |
Magnetic HA resin |
Thermo Fisher Scientific |
88836 |
Mini-PROTEAN TGX Stain-Free gels (4%–20%, 12-well comb, 20 μL) |
Bio-Rad |
4568095 |
TransIT-insect transfection reagent |
Mirus Bio |
10766-896 |
|
Experimental models: Cell lines |
|
Drosophila S2 cells |
ATTC |
CRL1963 |
|
Other |
|
0.45 μM PVDF membrane filter |
Milipore Sigma |
S2HVU05RE |
16-tube SureBeads magnetic rack |
Bio-Rad |
1614916 |