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Lysis Buffer
| Reagent | Final concentration | Amount from stock | Reagent stock |
|---|---|---|---|
| Tris pH 8 | 50 mM | 15 mL | 1 M |
| NaCl | 150 mM | 45 mL | 3 M |
| IGEPAL CA-630 | 1% | 3 mL | 100% |
| Glycerol | 10% | 30 mL | 100% |
| MG-132 Protease Inhibitor | N/A | N/A | 100% |
| ddH2O | N/A | 207 mL | 100% |
| Total | N/A | 300 mL | N/A |
