Reagent | Final concentration | Amount from stock | Reagent stock |
---|---|---|---|
Tris pH 8 | 50 mM | 15 mL | 1 M |
NaCl | 150 mM | 45 mL | 3 M |
Glycerol | 10% | 30 mL | 100% |
ddH2O | N/A | 210 mL | 100% |
Total | N/A | 300 mL | N/A |
Reagent | Final concentration | Amount from stock | Reagent stock |
---|---|---|---|
Tris pH 8 | 50 mM | 15 mL | 1 M |
NaCl | 150 mM | 45 mL | 3 M |
Glycerol | 10% | 30 mL | 100% |
ddH2O | N/A | 210 mL | 100% |
Total | N/A | 300 mL | N/A |