Table 5.
Mass spectrometry-based proteomic profiling of organellar and subcellular markers in aged mouse hindlimb muscle.
| Accession | Protein name | Gene | Peptides | Coverage (%) | Molecular mass (kDa) |
|---|---|---|---|---|---|
| (i) Sarcolemma | |||||
| Q8VDN2 | Na+/K+-ATPase, alpha-1 | Atp1a1 | 34 | 38.7 | 113.0 |
| Q6PIE5 | Na+/K+-ATPase, alpha-2 | Atp1a2 | 30 | 36.8 | 112.2 |
| Q9WV27 | Na+/K+-ATPase, alpha-4 | Atp1a4 | 7 | 7.8 | 114.9 |
| P14094 | Na+/K+-ATPase, beta-1 | Atp1b1 | 6 | 27.6 | 35.2 |
| P14231 | Na+/K+-ATPase, beta-2 | Atp1b2 | 8 | 32.8 | 33.3 |
| P97370 | Na+/K+-ATPase, beta-3 | Atp1b3 | 2 | 9.7 | 31.8 |
| Q9ESD7 | Dysferlin | Dysf | 27 | 18 | 237.9 |
| Q69ZN7 | Myoferlin | Myof | 34 | 21 | 233.3 |
| P82348 | Sarcoglycan, gamma | Sgcg | 2 | 11.3 | 32.1 |
| (ii) Caveolae | |||||
| P49817 | Caveolin-1 | Cav1 | 12 | 68 | 20.5 |
| Q9WVC3 | Caveolin-2 | Cav2 | 4 | 40.7 | 18.2 |
| P51637 | Caveolin-3 | Cav3 | 3 | 25.8 | 17.4 |
| (iIi) Organellar markers (ribosome, Golgi apparatus, peroxisome, proteasome, lysosome) | |||||
| P14206 | 40S ribosomal protein SA (ribosome | ) Rpsa | 18 | 63.1 | 32.8 |
| P55937 | Golgin-160 (Golgi apparatus) | Golga3 | 6 | 6.0 | 167.2 |
| P24270 | Catalase (peroxisome) | Cat | 30 | 63.9 | 59.8 |
| P61089 | Ubiquitin-conjugating enzyme E2 (proteasome) | Ube2n | 8 | 55.3 | 17.1 |
| P11438 | Lysosome-associated membrane glycoprotein 1 (lysomsome) | Lamp1 | 3 | 7.6 | 43.9 |
| (iv) Nucleus | |||||
| O08579 | Emerin (inner nuclear membrane) | Emd | 5 | 28.2 | 29.435 |
| P48678 | Lamin-A/C (nuclear lamina) | Lmna | 60 | 73.7 | 74.2 |
| P21619 | Lamin-B2 (nuclear lamina) | Lmnb2 | 15 | 24.8 | 67.3 |
| P10922 | Histone H1.0 | H1f0 | 5 | 27.3 | 20.9 |
| Q64522 | Histone H2A | Hist2h2ab | 8 | 66.2 | 14.0 |
| P02301 | Histone H3.3 | H3f3c | 7 | 47.1 | 15.3 |
| P62806 | Histone H4 | Hist1h4a | 12 | 60.2 | 11.4 |
| (v) Cytosol | |||||
| P13634 | Carbonic anhydrase CA1 | Ca1 | 2 | 10 | 28.3 |
| P00920 | Carbonic anhydrase CA2 | Ca2 | 3 | 22.7 | 29.0 |
| P16015 | Carbonic anhydrase CA3 | Ca3 | 17 | 85 | 29.4 |
| P06151 | Lactate dehydrogenase | Ldha | 23 | 74.4 | 36.5 |
| P09528 | Ferritin heavy chain | Fth1 | 10 | 62.1 | 21.1 |
| P29391 | Ferritin light chain 1 | Ftl1 | 6 | 48.6 | 20.8 |
| (vi) Neuromuscular junction, motor neuron and Schwann cells | |||||
| P13595 | Neural cell adhesion molecule NCAM1 | Ncam1 | 10 | 12.4 | 119.4 |
| P27573 | Myelin protein P0 | Mpz | 5 | 20.2 | 27.6 |
| P04370 | Myelin basic protein | Mbp | 4 | 17.6 | 27.2 |
| Q9CQW1 | Synaptobrevin | Ykt6 | 3 | 15.2 | 22.3 |
| O09117 | Synaptophysin | Sypl1 | 2 | 13 | 28.9 |