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. 1999 Jul;73(7):5833–5842. doi: 10.1128/jvi.73.7.5833-5842.1999

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Copyright © 1999, American Society for Microbiology

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FIG. 3 — HIV-1 infection of mucosal mononuclear cells I. (A) Detection of proviral HIV-1 DNA in sorted CD3⁺ T cells (T) and HLA-DQ^high CD14⁻ CD3⁻ DC. At 48 h after inoculation with DNase-treated HIV-1_Ba-L (donors 1 and 2) or HIV-1_Ba-L, HIV-1_M1, HIV-1_M2, HIV-1_LAI, and HIV-1_SF-2 (donor 3), DNA was isolated from equal numbers (10⁴) of cells and HIV-1 gag DNA copy numbers were measured by using a quantitative-competitive PCR enzyme immunoassay. Residual HIV-1 gag DNA copy numbers detected in the DNase-treated inocula (donor 1: Ba-L, 112 copies; donor 2: Ba-L, 0 copies; donor 3: Ba-L, 25 copies, M1, 0 copies, M2, 36 copies, LAI, 274 copies, and SF-2, 26 copies) were subtracted from the sample copy numbers. Error bars indicate standard deviations for duplicate infections in the third donor. (B) Reverse transcriptase (RT) activity in supernatants taken at various time points after infection of sorted CD3⁺ T cells (T, dashed lines) and CD3⁺ HLA-DQ^high T-cell–DC clusters (T-DC, solid lines) with either HIV-1_Ba-L or two primary genital mucosal HIV-1 isolates (HIV-1_M1 and HIV-1_M2). Infections were performed in duplicate, and the error bars indicate standard deviations. (C) HIV-1 p24 antigen concentrations measured in the same culture supernatants as in panel B. The upper limit of the assay was 3.7 ng/ml. (D) Comparison of reverse transcriptase activities in the supernatants of T and T-DC cultures from five mucosal donors after 5 to 6 days of infection with five different HIV-1 isolates.

FIG. 3 — HIV-1 infection of mucosal mononuclear cells I. (A) Detection of proviral HIV-1 DNA in sorted CD3⁺ T cells (T) and HLA-DQ^high CD14⁻ CD3⁻ DC. At 48 h after inoculation with DNase-treated HIV-1_Ba-L (donors 1 and 2) or HIV-1_Ba-L, HIV-1_M1, HIV-1_M2, HIV-1_LAI, and HIV-1_SF-2 (donor 3), DNA was isolated from equal numbers (10⁴) of cells and HIV-1 gag DNA copy numbers were measured by using a quantitative-competitive PCR enzyme immunoassay. Residual HIV-1 gag DNA copy numbers detected in the DNase-treated inocula (donor 1: Ba-L, 112 copies; donor 2: Ba-L, 0 copies; donor 3: Ba-L, 25 copies, M1, 0 copies, M2, 36 copies, LAI, 274 copies, and SF-2, 26 copies) were subtracted from the sample copy numbers. Error bars indicate standard deviations for duplicate infections in the third donor. (B) Reverse transcriptase (RT) activity in supernatants taken at various time points after infection of sorted CD3⁺ T cells (T, dashed lines) and CD3⁺ HLA-DQ^high T-cell–DC clusters (T-DC, solid lines) with either HIV-1_Ba-L or two primary genital mucosal HIV-1 isolates (HIV-1_M1 and HIV-1_M2). Infections were performed in duplicate, and the error bars indicate standard deviations. (C) HIV-1 p24 antigen concentrations measured in the same culture supernatants as in panel B. The upper limit of the assay was 3.7 ng/ml. (D) Comparison of reverse transcriptase activities in the supernatants of T and T-DC cultures from five mucosal donors after 5 to 6 days of infection with five different HIV-1 isolates.