Fig. 1.

MAP3K8 activation and silencing in A549 cells modulate IL-1β-induced cytokine expression. a)1 ng/ml of IL-1β induced MAP3K8/GAPDH mRNA expression. in A549 cells, b)MAP3K8 gene expression in A549, BEAS-2B and NHBE cells at 2 h after IL-1β stimulation, c) MAP3K8 phosphorylation (P-MAP3K8) protein expression induced by IL-1β in A549 cells, d) siRNA knockdown of MAP3K8, including reagent control, GAPDH positive control, MAP3K8 gene and protein knockdown efficiency at 2 h and 48 h, respectively e, f)MAP3K8 knockdown reduces IL-6 and IL-8 mRNA expression at 2, 4 and 6 h, and their protein expression at 8 and 24 h