Fig. 2.

MAP3K8 regulates MAPK signalling pathways of ERK/MAPK and SAPK/JNK but not the p38 MAPK. a)RNAi-mediated knockdown of MAP3K8 results in consistent reduction of MAP3K8 protein expression in the presence of 1 ng/ml IL-1β, assessed at 20 and 30 min after IL-1β stimulation, b) IL-1β-activated MAP3K8 regulates the phosphorylation of ERK1/2 and SAPK/JNK, but not p38 at 20 and 30 min after IL-1β stimulation, c) IL-1β-activated MAP3K8 regulates the phosphorylation of MEK1/2 and MKK4 but not MKK7 in A549 cells, MAPK phosphorylation was assessed and compared between non-targeting siRNA and MAP3K8 siRNA group. Data are expressed as the mean ± SEM of at least three separate experiments. The gels show repeat labelling of Western blots with different antibodies: each section represents an individual blot, with each antibody stain in a separate box