Fig. 3.

MAP3K8 knockdown enhances dexamethasone suppression of IL-1β-induced cytokine production in A549 cells. a)MAP3K8 knockdown reduces IL-8 and b) RANTES supernatant release from A549 cells pre-treated with dexamethasone at 24 h after 1 ng/ml IL-1β stimulation, c) Dexamethasone effects on phosphorylation of ERK/MAPK and SAPK/JNK but not their upstream MEK1/2 or MKK4 analysed by Western blotting at 30 min after IL-1β stimulation, d) MAP3K8 knockdown enhances the inhibition of phosphorylated ERK1/2 and SAPK/JNK but not p38 MAPK at 30 min after IL-1β stimulation in the presence of dexamethasone (µM) pre-treatment. (All mean ± SEM of at least three separate experiments). The gels show repeat labelling of Western blots with different antibodies