Table 1.
Genes altered by siRNA knockdown of MAP3K8 in A549 cells after IL-1β stimulation of A549 cells
| ID | Dif1 | Dif2 | Dif4 | Dif6 | Dif8 | Dif12 | Ave Expr | adj. P.Val | Gene Assignment | Function Group |
|
|---|---|---|---|---|---|---|---|---|---|---|---|
| 8,149,825 | -0.0297 | -0.5986 | -0.7126 | 0.2788 | 0.3121 | 0.2948 | 9.7303 | 7.78E-12 | STC1 | Growth factors | |
| 7,926,900 | -0.0312 | -0.6082 | -0.1792 | 0.1979 | 0.0593 | 0.2146 | 9.1270 | 2.17E-06 | MAP3K8 | ERK pathways | |
| 8,139,207 | -0.3252 | -0.6912 | -0.3870 | 0.0652 | 0.0955 | 0.3487 | 6.6862 | 1.72E-05 | INHBA | Growth factors | |
| 8,029,693 | -0.5578 | -1.3920 | 1.2786 | 0.3235 | 0.1937 | -0.2268 | 6.5645 | 1.88E-05 | FOSB | ERK pathways | |
| 7,951,284 | 0.2147 | -0.2445 | -0.9981 | -0.0006 | -0.0489 | 0.8034 | 4.4087 | 0.00010247 | MMP3 | Extracellular proteases | |
| 8,150,509 | 0.2576 | -0.4648 | 0.1007 | 0.2679 | 0.1877 | 0.2294 | 9.0473 | 0.0002872 | PLAT | Extracellular proteases | |
| 7,951,259 | 0.5534 | -0.9547 | -0.5190 | 0.2045 | -0.3736 | 0.3734 | 6.3240 | 0.00045375 | MMP10 | Extracellular proteases | |
| 8,153,201 | 0.0479 | -0.4685 | -0.1152 | -0.0437 | 0.3042 | -0.2654 | 10.4016 | 0.00045375 | EIF2C2 | ||
| 7,917,649 | 0.2127 | 0.0377 | 0.1270 | -0.3302 | -0.2098 | -0.1283 | 8.7129 | 0.00093737 | TGFBR3 | Growth factors | |
| 7,944,769 | -0.0572 | -0.0887 | -0.2799 | -0.0066 | 0.3259 | 0.1219 | 10.4395 | 0.0009716 | GRAMD1B | ||
| 8,095,728 | 0.1828 | -0.2296 | -0.2194 | -0.1545 | 0.0599 | 0.3256 | 11.6033 | 0.00175164 | EREG | Growth factors | |
| 7,996,772 | -0.0158 | 0.0041 | -0.0028 | -0.2563 | -0.0490 | -0.0799 | 9.8132 | 0.00259827 | SLC7A6 | ||
| 8,154,245 | -0.4487 | 0.2877 | 0.0338 | 1.1178 | -0.4189 | -0.2688 | 5.9701 | 0.00317684 | PDCD1LG2 | ERK pathways | |
| 8,124,166 | 0.1576 | -0.0397 | 0.2982 | 0.2472 | -0.2132 | 0.0147 | 7.8654 | 0.005414 | MBOAT1 | Lysophopholipids | |
| 7,957,298 | -0.1566 | -0.0458 | -0.3356 | 0.0307 | 0.2926 | 0.0490 | 9.7235 | 0.00550053 | NAV3 | ||
| 7,951,271 | -0.3148 | -0.4711 | -0.5804 | 0.1105 | -0.1583 | 0.5121 | 7.2633 | 0.00550053 | MMP1 | Extracellular proteases | |
| 8,091,600 | 0.1084 | -0.0836 | 0.1036 | 0.4321 | -0.1186 | -0.0600 | 7.4795 | 0.00550053 | PLCH1 | Lysophopholipids | |
| 7,902,227 | 0.0436 | 0.2069 | 0.3442 | 0.0419 | -0.5557 | -0.0956 | 7.0090 | 0.00998721 | GADD45A | ERK pathways | |
| 8,057,677 | 0.0052 | 0.1962 | -0.0289 | -0.4795 | 0.0687 | -0.0085 | 8.1113 | 0.00998721 | SLC40A1 | Iron metabolism | |
| 7,997,401 | 0.0344 | -0.1522 | 0.1799 | 0.2824 | 0.2857 | -0.0481 | 7.6882 | 0.01063101 | BCMO1 | ||
| 8,054,722 | 0.2205 | -0.9733 | -0.1205 | -0.0332 | 0.2151 | 0.5247 | 6.2915 | 0.01063101 | IL1B | ||
| 8,152,617 | 0.0279 | -0.1290 | -0.3494 | -0.2234 | 0.2312 | 0.2599 | 8.8467 | 0.01211707 | HAS2 | Extracellular matrix | |
| 8,123,609 | 0.0341 | -0.5227 | -0.0309 | 0.1276 | 0.0553 | 0.1139 | 10.1999 | 0.01306179 | SERPINB9 | Extracellular matrix | |
| 7,961,371 | 0.0566 | -0.2009 | -0.1672 | -0.0378 | -0.0165 | 0.0102 | 9.8130 | 0.01434946 | DUSP16 | ERK pathways | |
| 7,909,214 | 0.1965 | -0.1592 | 0.0568 | 0.6090 | 0.1808 | -0.4726 | 7.2441 | 0.01434946 | RASSF5 | ERK pathways | |
| 7,908,072 | 0.1938 | -0.2754 | -0.2798 | 0.1108 | -0.0594 | 0.1371 | 9.7632 | 0.01434946 | LAMC2 | Extracellular matrix | |
| 8,131,666 | -0.1251 | -0.4561 | 0.0721 | -0.0613 | -0.0307 | -0.0677 | 8.2473 | 0.01434946 | ITGB8 | Extracellular matrix | |
| 8,146,579 | 0.0335 | 0.0855 | -0.0022 | -0.2335 | -0.1355 | -0.0524 | 7.9175 | 0.01434946 | CHD7 | ||
| 8,162,940 | -0.1683 | 0.1241 | -0.0298 | -0.1565 | -0.0990 | -0.2986 | 8.7714 | 0.01759226 | ABCA1 | ||
| 8,160,521 | 0.0144 | 0.0895 | 0.1278 | 0.3431 | -0.2911 | -0.1801 | 8.5845 | 0.01906981 | MOB3B | ||
| 8,021,635 | 0.1217 | 0.0319 | -0.4090 | -0.3232 | 0.3902 | 0.1589 | 3.6854 | 0.02025975 | SERPINB2 | Extracellular matrix | |
| 7,987,454 | 0.1111 | 0.0995 | 0.3137 | -0.5803 | -0.1220 | -0.1906 | 7.2998 | 0.02025975 | BMF | ||
| 8,040,365 | 0.0105 | 0.0497 | -0.2679 | 0.0267 | -0.7976 | 0.7653 | 4.1035 | 0.0209142 | TRIB2 | ||
| 7,951,309 | -0.0491 | -0.1362 | -0.6825 | 0.1977 | 0.0703 | 0.2436 | 3.9447 | 0.02471666 | MMP13 | Extracellular proteases | |
| 8,072,678 | 0.1496 | -0.0771 | -0.3122 | 0.0068 | 0.5784 | -0.1840 | 9.9875 | 0.02655837 | HMOX1 | Iron metabolism | |
| 8,073,214 | -0.0930 | 0.0348 | -0.0701 | -0.2201 | 0.0692 | -0.2012 | 8.1931 | 0.02655837 | TNRC6B | ||
| 7,909,332 | -0.1267 | 0.0167 | -0.1388 | -0.2643 | -0.0820 | 0.4067 | 10.1092 | 0.02655837 | CD55 | ||
| 7,956,009 | -0.0204 | 0.0449 | -0.1819 | 0.0758 | 0.4650 | 0.1654 | 8.1114 | 0.02655837 | METTL7B | ||
| 7,917,347 | 0.0433 | -0.0119 | 0.2583 | -0.1106 | -0.2745 | -0.2573 | 9.1879 | 0.03022086 | DDAH1 | ||
| 8,132,694 | -0.1352 | 0.0592 | -0.1252 | -0.3574 | 0.2755 | 0.2553 | 9.9915 | 0.03722356 | IGFBP1 | Growth factors | |
| 7,954,077 | 0.0697 | 0.0799 | -0.0287 | -0.1959 | -0.2314 | -0.1436 | 8.0405 | 0.03868964 | KIAA1467 | ||
| 8,121,257 | 0.0055 | -0.3574 | -0.0367 | 0.2346 | -0.0094 | -0.3641 | 8.7712 | 0.04079342 | PRDM1 | ||
| 8,007,931 | -0.0643 | -0.0290 | 0.0077 | -0.3214 | -0.1882 | 0.0141 | 6.1123 | 0.04321407 | ITGB3 | ||
| 7,946,478 | -0.0081 | -0.0778 | -0.1677 | -0.1382 | 0.4313 | -0.2799 | 9.9379 | 0.04328626 | DENND5A | ||
| 7,922,229 | -0.1620 | -0.7991 | -0.0055 | 0.6619 | 0.2436 | 0.0700 | 5.4737 | 0.04535622 | SELE | ||
| 8,129,861 | -0.0997 | -0.1270 | -0.1209 | -0.2175 | 0.0590 | -0.0593 | 9.2467 | 0.04811681 | IFNGR1 | ||
Ave. Expr: average expression; adj.P. Val: adjust P value. Dif1 – Dif12 are the log2 fold changes associated with each contrast. The contrasts were specified as follows (where Knockdown/Control are the conditions, and 0–12 are the time points of the hours after stimulation):
Dif1=(Knockdown.1 h-Knockdown.0 h)-(Control.1 h-Control.0 h);
Dif2=(Knockdown.2 h-Knockdown.1 h)-(Control.2 h-Control.1 h);
Dif4=(Knockdown.4 h-Knockdown.2 h)-(Control.4 h-Control.2 h);
Dif6=(Knockdown.6 h-Knockdown.4 h)-(Control.6 h-Control.4 h);
Dif8=(Knockdown.8 h-Knockdown.6 h)-(Control.8 h-Control.6 h);
Dif12=(Knockdown.12 h-Knockdown.8 h)-(Control.12 h-Control.8 h).