FIG. 5.

Chemokine receptor expression in AM assessed by Northern analysis. RNA obtained from AM of three individuals (lanes A, B, and C) and, as a control, from PBM and PBL from individual A. The PBM and PBL were purified with immunomagnetic beads coated with antibodies against CD14 (PBM) and CD3 (PBL), respectively. The cells were analyzed with specific probes for CCR5, CXCR4, and CCR2b. From top to bottom are shown expression of CCR5, CXCR4, CCR2b, and GAPDH. Lanes: 1 to 3, AM for three healthy individuals; 4, PBM from individual A; 5, PBL from individual A. The sizes of the mRNAs are indicated in kilobases (kb).