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Overview of the neuronal tau protein and the analytical features. (a) Schematic of tau protein 2N4R isoform. Indicated are the N1 and N2 inserts, the proline-rich region (P1 & P2), the MTBR (R1-R4), the epitopes of the four antibodies used, the detected phospho-sites, and both the tryptic non-phospho and phospho peptides quantified. (b) Details of the isoform specific regions highlighting the quantified isoform specific tryptic peptides. (c) The fraction/antibody combinations analysed
