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. 2024 Jul 19;19:54. doi: 10.1186/s13024-024-00741-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Selected scatter-plots of quantified tryptic phospho-peptides after IP with HT7. Shown are abundancies of peptides carrying one (p1, upper pannels), two (p2, middle panels), or three (p3, bottom panels) phosphorylations. Data is grouped according to fractions. TBS fractions are indicated in blue and SI fractions in pink. Panel top: box/scatter plots with medians, inter-quartile intervals, and min/max intervals indicated. Fold changes of the mean values compared with the controls in respective fraction are indicated for each disease group. Panel bottom: significances according to one-way ANOVA not assuming homoscedasticity (Brown-Forsythe/Welch) followed by unpaired t-test with Welch’s correction. Calculations are only performed within each fraction, upper right (blue) for TBS and lower left (pink) for SI; ns not significant (p ≥ 0.05), * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, – test not possible to perform due to zero variance in one of the groups compared. See Suppl. Figure 7 for all antibody-fraction combinations analysed