FIG. 2.
Immunoblotting analysis of c-Myb mutant-transformed chicken yolk sac cells. From the above yolk sac assays, v-Myb, c-Myb, and c-Myb mutant-transformed yolk sac cells were collected and subjected to immunoblot analysis. All yolk sac cell lysates were derived from 5 × 105 cells with the exception of primary yolk sac cells obtained from a 13-day-old chicken embryo (6 × 106 cells/lane) and CCC-dPS (3 × 105 cells/lane). The cell lysates were resolved on an SDS–10% PAGE gel and immunoblotted with Myb antibodies (5E and 2.7). The relative mobilities of protein markers are indicated in kilodaltons.