FIG. 3.

Morphology of yolk sac cells transformed by dGE, CCC, CCd, and CCC-dPSBN. The morphologies of a representative set of transformed yolk sac cells are shown. The yolk sac cells from liquid culture outgrowth were cytocentrifuged onto glass slides and stained with Diff-Quick (DADE). The v-Myb-transformed cells (dGE) are a homogeneous population of monoblasts. The cells transformed by c-Myb (CCC), a double deletion mutant of c-Myb (CCC-dPSBN), and a C-terminal truncation of c-Myb (CCd) exhibited heterogeneous phenotypes. Cells of one type (marked with arrowheads) resemble granulocytes with distinct cytoplasmic granules. The rest of the cells are macrophage-like and have an eccentric nucleus with many vacuoles in the cytoplasm.