TABLE 1.

Transforming activities of c-Myb mutants

Construct	No. of cells/ml in expta		Frequency of liquid culture outgrowthb	Relative colony no.c		Transformation leveld
	1 (day 23)	2 (day 22)		Avg	ADe
N-Cla (vector)	<103	<103	0/4	0	0	−
dGE (v-Myb)	1.4 × 107	9 × 106	4/4	100	0	+++++
CCC (c-Myb)	<103	4.1 × 104	2/4	1.8	1.2	+
CCd	2 × 104	1 × 104	4/4	2.5	1.1	+
CCC-dMN	<103	<103	0/4	0.5	0.4	−
CCC-dNN	<103	<103	0/4	3.1	2.5	−/+
CCC-dBN	1.5 × 104	1 × 104	3/4	13.3	3.6	++
CCC-dPSBB	6 × 104	1.6 × 104	4/4	11.6	5.5	++
CCC-dPSBN	1.6 × 105	1.1 × 105	4/4	37.4	17.1	+++
CCC-dSB	3.5 × 104	2 × 104	2/4	4.7	1.0	+
CCC-dSH	0.7 × 104	1.4 × 104	2/4	15.2	5.8	++
CCC-dPS	3 × 104	4.8 × 104	2/4	8.8	8.0	++
CCC-dBH	2 × 104	3.8 × 104	4/4	16.0	7.0	++
CCC-304GP	<103	<103	0/4	0	0	−
CCC-389GPN	2 × 104	<103	1/4	2.9	0.9	+
CCC-304GP/389GPN	<103	<103	0/4	NDf	ND	−

^aThe cell numbers were determined on the day indicated after cocultivation. 

^bThe frequency of liquid culture outgrowth is from three yolk sac assays and one bone marrow assay. 

^cIn methyl cellulose assays, relative colony numbers were obtained by assigning the colony number of N-dGE (v-Myb) a value of 100. Averages of relative colony numbers were from three independent methylcellulose assays. 

^dThe nontransforming viruses and v-Myb are designated arbitrarily − and +++++, respectively. The transforming activities of c-Myb and c-Myb mutants were determined relative to v-Myb activity after considering cell number count, frequency of liquid culture outgrowth, and colony number count in the methylcellulose assay. 

^eAD, average deviation. 

^fND, not done.