TABLE 2.
Phenotypes of cells transformed by c-Myb mutants
| Construct | Morphologya | Expression of cell surface markerb
|
||
|---|---|---|---|---|
| 1C3 | HLO72 | Mim-1 | ||
| N-Cla (vector) | ND | ND | ND | ND |
| dGE (v-Myb) | Monocytic | − | + | − |
| CCC (c-Myb) | Bilineage | + | + | + |
| CCd | Bilineage | + | + | + |
| CCC-dMN | ND | ND | ND | ND |
| CCC-dNN | ND | ND | ND | ND |
| CCC-dBN | Bilineage | + | + | + |
| CCC-dPSBB | Bilineage | + | + | + |
| CCC-dPSBN | Bilineage | + | + | + |
| CCC-dSB | Bilineage | + | + | + |
| CCC-dSH | Bilineage | ND | ND | ND |
| CCC-dPS | Bilineage | + | + | + |
| CCC-dBH | Bilineage | + | + | + |
| CCC-304GP | ND | ND | ND | ND |
| CCC-389GPN | Granulocytic | ND | ND | ND |
| CCC-304GP/389GPN | ND | ND | ND | ND |
a
Cell morphology was determined by staining cytospins with Diff-Quick. The bilineage phenotype is based on the fact that the transformed cells display heterogeneous populations with both monocytic and granulocytic features. ND, not determined.
b
The expression of cell type-specific markers was determined by FACS analysis with 1C3 (granulocytic lineage) and HLO72 (monocytic lineage) antibodies and by immunoblotting with an anti-Mim-1 antibody (promyelocytic or granulocytic lineage).