Fig. 4.

Circ_0007386 acts as a sponge for miR-383-5p. (A) Relative luciferase activity of the circ_0007386 wild-type (circ_0007386 WT) in 293 A cells transfected with 15 candidate miRNAs mimics or mimic NC. (B) Schematic of the luciferase reporter plasmids of circ_0007386 WT and mutant-type (circ_0007386 MUT). (C) Luciferase activity of circ_0007386 WT and circ_0007386 MUT transfected with miR-383-5p mimic or mimic NC. (D) Relative expression levels of miR-383-5p were determined using RT-qPCR in various cell lines of NSCLC. E-F. Relative expression of miR-383-5p in NSCLC tissues and adjacent nontumor tissues was analyzed using RT-qPCR (n = 80). G. Relative expression of miR-383-5p in A549 and H1299 cells, transfected as indicated, was detected by RT-qPCR. H. FISH assay showed that miR-383-5p (Cy3-labeled probe) was abundant in the cytoplasm, and DAPI was used to stain the nucleus. Images are shown at 600× magnification. Scale bar = 20 μm. I. RT-qPCR of the relative expression levels of miR-383-5p in A549 and H1299 cells transfected with the miR-383-5p mimics or inhibitor or its controls. J-L. Rescue experiments, including CCK8 and colony formation were conducted in cells treated with four different conditions (si-NC + inhibitor NC, si-circ_0007386 + inhibitor NC, si-NC + miR-383-5p inhibitor, si-circ_0007386 + miR-383-5p inhibitor). M-P. Rescue experiments, including TUNEL staining and flow cytometry assays were conducted in A549 and H1299 cells in the indicated groups. Images are shown at 400× magnification. Scale bar = 40 μm. Data are shown as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001