Fig. 5.

CIRBP is a target gene of miR-383-5p and indirectly regulated by circ_0007386. A. Venn diagram showing 65 genes that are potential targets of miR-383-5p predicted by three databases (Targetscan, miRDB, and ENCORI). B. The expression of CIRBP mRNA in NSCLC cells was assessed using RT-qPCR. C-D. The relative expression levels of CIRBP were compared between NSCLC tissues and adjacent nontumor tissues (n = 80). E. IHC staining of CIRBP expression in xenograft tumors in four different groups (circ_0007386 overexpression construct (pLCDH-circ_0007386), control vector (pLCDH-vector), circ_0007386 shRNA (sh-circ_0007386) and control shRNA (sh-NC)). Images are shown at 400× magnification. Scale bar = 50 μm. F. Schematic of the luciferase reporter plasmids for CIRBP wild-type (CIRBP WT) and mutant-type (CIRBP MUT). G. Luciferase activity of the CIRBP WT and CIRBP MUT reporters in 293 A cells transfected with miR-383-5p mimics or mimic NC. H-I. RT-qPCR and western blot of CIRBP mRNA and protein levels in cells transfected with miR-383-5p mimics and inhibitor and its controls. J-M. Relative expression of CIRBP at both the mRNA and protein levels in the indicated groups (si-NC + NC-inhibitor, si-circ_0007386 + NC-inhibitor, si-circ_0007386 + miR-383-5p inhibitor and vector + mimics NC, circ_0007386 + NC-mimics, circ_0007386 + miR-383-5p mimics). Data are shown as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001