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. 2024 Jul 20;43:200. doi: 10.1186/s13046-024-03116-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing. (A) Western blot analysis of HIF1α expression in cells under hypoxia or normoxia. (B) circ_0007386 expression under hypoxia and normoxia analyzed using RT-qPCR. (C) YAP1 expression under hypoxia and normoxia analyzed by RT-qPCR. (D) The relative expression of CRIM1 mRNA and circ_0007386 after YAP1 overexpression under hypoxia and normoxia were detected by RT-qPCR. (E) Venn diagram showing RBPs that bind to the CRIM1 pre-mRNA transcript predicted by three databases (CircInteractome, CircFunBase, and ENCORI). (F) Relative expression of circ_0007386 in A549 and H1299 cells transfected with the indicated vectors or siRNAs was determined using RT-qPCR. (G) RIP assays using an EIF4A3 antibody were performed to examine interactions with CRIM1 mRNA. (H) CoIP analysis of the YAP1-EIF4A3 interaction in cells treated as indicated. (I) RIP assays using an EIF4A3 antibody were performed to examine interactions with CRIM1 mRNA under hypoxia and normoxia. Data are shown as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001