FIG. 1.

Expression of ORF 59 protein (PF-8) and ORF K8.1 protein (gpK8.1) in the BCBL-1 cell line stimulated with TPA or n-butyric acid. Cells were stimulated with 30 nM TPA or 0.3 mM n-butyric acid for 2 days. Cellular protein was isolated, separated by gel electrophoresis, and blotted onto nitrocellulose membranes. PF-8 (A) and gpK8.1 (B) were labeled with protein-specific MAbs, coupled to alkaline phosphatase, and visualized with Western Blue. Lanes: 1, unstimulated cells; 2, TPA-stimulated cells; 3, n-butyric acid stimulated cells. Data shown are representative of five experiments.