TABLE 3.

Viral titers in organs from LCMV-infected perforin-deficient and -competent NP transgenic or nontransgenic mice that survived long-term^a

Type of mouse and time postinfection	Viral titer (PFU/g of tissue)
	Liver	Kidney	Pancreas	Spleen
Day 7
p+/− NP−	2 × 102 ± 70	ND	ND	ND
p+/− NP+	NDb	ND	ND	ND
p−/− NP−	0.8 × 105 ± 3 × 104	2 × 103 ± 1 × 103	1 × 103 ± 400	7 × 104 ± 4 × 104
p−/− NP+	5 × 104 ± 2 × 104	5 × 102 ± 200	2 × 102 ± 150	1.5 × 105 ± 6 × 104
Day 60
p+/− NP−	ND	ND	ND	ND
p+/− NP+	ND	ND	ND	ND
p−/− NP−	1 × 105 ± 4 × 104	1.4 × 104 ± 4 × 103	2 × 104 ± 1 × 104	3 × 104 ± 2 × 104
p−/− NP+	1 × 105 ± 7 × 104	1 × 104 ± 5 × 103	1.6 × 104 ± 1 × 104	3 × 104 ± 1.1 × 104

^aThree or four mice in each group were infected with 10⁵ PFU of LCMV intraperitoneally. Viral titers in the spleen, liver, thymus, kidney, pancreas and brain were determined by a plaque assay at 7 or 60 days postinfection. No significant differences were found between thymic expressors and nonexpressors. Brains and thymuses of all perforin-deficient mice also contained virus as assessed by direct immunohistochemistry (data not shown). 

^bND, nondetected.