TABLE 4.
CD8, TNF-α, and IFN-γ mRNA content in organs of perforin-deficient and -competent NP expressors and nonexpressorsa
| Type of mouse and time post-infection | Relative mRNA contentb
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Liver
|
Kidney
|
Spleen
|
|||||||
| CD8 | TNF-α | IFN-γ | CD8 | TNF-α | IFN-γ | CD8 | TNF-α | IFN-γ | |
| Day 7 | |||||||||
| p+ NP− | 8.2 | 1.4 | 4.3 | 2.4 | 0.9 | 1.3 | 1.7 | 0.9 | 4.5 |
| p+ NP+ | 6.2 | 1.4 | 3.6 | 2.2 | 0.9 | 3.7 | 1.4 | 0.9 | 3.8 |
| p− NP− | 42.3 | 3.9 | 99.9 | 4.8 | 1.2 | 19.5 | 4.8 | 2.0 | 59.3 |
| p− NP+ | 32.2 | 3.9 | 133 | 5.0 | 0.8 | 15.5 | 3.2 | 2.4 | 55.1 |
| Day 60 | |||||||||
| p− NP− | 13.1 | 1.7 | 25 | 11.6 | 1.2 | 82.2 | 6.4 | 1.9 | 10 |
| p− NP+ | 2.6 | 1.3 | 1.5 | 3.2 | 0.86 | 4.9 | 0.72 | 1.0 | 2.9 |
a
Total RNA extracted from the indicated organs of two representative mice per group was analyzed by the RNase protection assay for CD8, TNF-α, and IFN-γ RNA contents (Fig. 2 and 4) and quantitated by phosphorImager analysis.
b
CD8, TNF-α, and IFN-γ RNA values were normalized to the values of the housekeeping gene L32, divided by the respective RNA values detected in uninfected controls, and expressed as mean values, thus representing fold increase relative to L32 controls. Boldfaced values reflect important differences between perforin-deficient thymic expressors and nonexpressors.