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Generation of a VP6-knockout rotavirus. (A) Generation of MA104 cells stably expressing VP6. VP6 expression was confirmed by Western blotting. (B) Growth of rSA11-VP6-KO. MA104-VP6 cells were infected with viruses at an MOI of 0.001 and then harvested at 72 hours post-infection. (C) Expression of viral proteins by rSA11-VP6-KO. Cells were infected with the virus at an MOI of 1.0. At 8 hours post-infection, cells were fixed and examined in an immunofluorescence assay.
