Fig 3.
Generation of single-round infectious rotaviruses expressing foreign genes. (A) Construction of the VP6 and NSP1 gene segments used to generate foreign gene-expressing rotaviruses. The number of nucleotides (nt) or amino acid residues (aa) is shown above. (B) Growth of the rescued recombinant rotavirus. MA104-VP6 cells were infected with viruses at an MOI of 0.001 and then harvested at 72 hours post-infection. (C) Fluorescence image of ZsG expression by rSA11-169–174Linker-ZsG. Cells were infected with viruses at an MOI of 1.0. At 8 hours post-infection, cells were fixed and examined in an immunofluorescence assay. (D) Kinetics of NLuc expression by rSA11-169–174Linker-NLuc. Cells were infected with viruses at an MOI of 0.01. The cells were harvested at the designated times. (E) NLuc-based neutralization test using serum from a mouse immunized with rSA11.