Fig 6.

Immunization of mice with the single-round infectious rotavirus. (A) Schematic showing the immunization protocol. Three groups of 4-week-old BALB/c mice (n = 5–7/group) were orally immunized with 1 × 10⁷ FFU of viruses. (B and C) Detection of serum IgG and IgA against rotavirus. Statistical significance was analyzed using a t-test. P values < 0.05 were considered statistically significant. ns = not significant. (D) Neutralization titer in serum from immunized mice. The neutralization test was performed using rSA11-NLuc. The neutralizing titer is calculated as the maximum serum dilution yielding more than 50% neutralization. The limit of detection (LOD) was 1:200. Neutralization titers below the detection limit are plotted as half of the detection limit (1:100). Statistical significance was analyzed using the Mann–Whitney test. P values < 0.05 were considered statistically significant. ns = not significant.