Fig 3.

Bronchoalveolar lavage (BAL) samples were obtained for ELISA testing, and lungs were obtained for subsequent immunohistochemistry at 3 dpi and were analyzed with nested 1-way ANOVA. MA10-SARS-CoV-2 infection increased the release of the inflammatory cytokine IL-6, and this IL-6 release was attenuated by the CGRP signaling blockade. In both C57BL/6J (A) and 129S (B) mouse strains, IL-6 release was attenuated by olcegepant SQ release. Moreover, in αCGRP-null mice (αCGRP-KO), IL-6 cytokine release was statistically equivalent to infected controls given olcegepant and uninfected controls. Significance is listed as *= P < 0.05, ** =P < 0.01, and ***= P < 0.001, and the italicized t represents P-value < 0.1.