Fig 4.

Older (18-month old) female and male mice were tested for weight loss and core temperature decreases after infection with MA-10 SARS-CoV-2, 10⁵ pfu virus at 0–7 dpi using two-way mixed-effects models and Bonferroni multiple comparisons test. At each dpi, asterisks correspond to comparisons between placebos versus olcegepant in infected mice since no differences were observed due to olcegepant in uninfected controls (A and C) We found that animals treated with olcegepant recovered from the initial weight loss, with male mice showing increased protection. (A) C57BL/6J; (C) 129/SvEv. (E) Like the IL-6 findings (Fig. 3), mice lacking the CGRP peptide (αCGRP-KO) were not significantly different from uninfected controls. (B, D, F) Viral infection caused detected core temperatures of mice to decrease, and this effect was unaltered by olcegepant treatment. P-values: *= P < 0.05, ** =P < 0.01, and ***= P < 0.001, and t represents a comparison with P-value < 0.1.