Fig 4. Pre-administration of metformin 3 days before irradiation significantly decreases the number of micronuclei in murine bone marrow cells and the number of DNA damage foci in colon and lung tissues, increasing mice survival after exposure to ionizing radiations.

(A) Metformin 200 mg/kg pre-administration significantly decreases the number of micronuclei in mice bone marrow cells, compared to the 7.5 cGy control, in 129/sv mice one-way ANOVA, **p<0.01, ****p<0.0001). (B, C) Pre-administration of metformin 200 mg/kg in mice for three consecutive days, decreases the number of 53BP1 foci of mice irradiated at the lethal dose of 10 Gy (one-way ANOVA, **p<0.01, ***p<0.001). (D) Long-term survival of wild type 129/sv mice feed with metformin chow 200 mg/kg for three consecutive days and then irradiated at the lethal dose of 10 Gy of X-rays (log-rank test, Chi square 7.1, **p<0.01).