FIG. 7.
Colocalization of MHV RNA and gene 1 products with the Golgi apparatus in HeLa-MHVR cells. (A) Mock-infected cells were stained with the D3 antiserum; (B and C) mock-infected cells were double stained with WGA (Golgi) (B) and the GRP78 antibody (ER) (C); (D to F) MHV-A59-infected cells were permeabilized and labeled with BrUTP, followed by double staining with the BrdU antibody (RNA) (D) and WGA (E); (G to I) virus-infected cells were double stained with the D3 antiserum (G) and WGA (H); (J to L) virus-infected cells were double stained with antibodies against D3 (J) and GRP78 (ER) (K). Panels F, I, and L are the superimposed images of D and E, G and H, and J and K, respectively.