FIG. 8.

Partial colocalization of MHV gene 1 products with the ER in 17CL-1 cells. (A) Mock-infected cells were stained with the D₃ antiserum; (B and C) mock-infected cells were double stained with WGA (Golgi) (B) and the GRP78 antibody (ER) (C); (D to F) MHV-A59-infected cells were double stained with the D₃ antiserum (D) and WGA (Golgi) (E); (G to I) virus-infected cells were double stained with antibodies against D₃ (G) and LAMP-1 (lysosome) (H); (J to L) virus-infected cells were double stained with antibodies against D₃ (J) and GRP78 (ER) (K); (M to O) MHV-A59-infected cells were permeabilized and labeled with BrUTP, followed by double staining with antibodies against BrdU (RNA) (M) and GRP78 (ER) (N). Panels F, I, L, and O are the superimposed images of D and E, G and H, J and K, and M and N, respectively.