Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| eFluor™ 450 anti-mouse CD3e (Clone 145-2C-11) | eBioscience | Cat#48-0031-82; RRID: AB_10735092 |
| eFluor™ 450 anti-mouse CD19 (Clone eBio1D3) | eBioscience | Cat#48-0193; RRID: AB_2043815 |
| eFluor™ 450 anti-mouse CD5 (Clone 53-7.3) | eBioscience | Cat#48-0051-82; RRID: AB_1603250 |
| eFluor™ 450 anti-mouse Ly-6G/Ly-6C(Gr-1) (Clone RB6-8C5) | eBioscience | Cat#48-5931-82; RRID: AB_1548788 |
| eFluor™ 450 anti-mouse CD11b (Clone M1/70) | eBioscience | Cat#48-0112-82; RRID: AB_1582236 |
| eFluor™ 450 anti-mouse CD11c (Clone N418) | eBioscience | Cat#48-0114-82; RRID: AB_1548654 |
| eFluor™ 450 anti-mouse NK1.1 (Clone PK136) | eBioscience | Cat#48-5941-82; RRID: AB_2043877 |
| eFluor™ 450 anti-mouse TER119 (Clone TER-119) | eBioscience | Cat#48-5921-82; RRID: AB_1518808 |
| eFluor™ 450 anti-mouse CD45R(B220) (Clone RA3-6B2) | eBioscience | Cat#48-0452-82; RRID: AB_1548761 |
| PE-Cyanine7 anti-mouse CD127(Clone A7R34) | eBioscience | Cat#25-1271-82; RRID: AB_469649 |
| APC-Cyanine7 anti-mouse CD90.2(Clone 30-H12) | Biolegend | Cat#105328; RRID: AB_10613280 |
| Super Bright™ 645 anti-mouse CD4(Clone RM4-5) | eBioscience | Cat#64-0042-82; RRID: AB_2662401 |
| Brilliant Violet 650™ anti-mouse CD45.1(Clone A20) | Biolegend | Cat#110736; RRID: AB_11124743 |
| Brilliant Violet 650™ anti-mouse CD179(c-Kit) (Clone ACK2) | Biolegend | Cat#135125; RRID: AB_2562446 |
| Brilliant Violet 785™ anti-mouse CD196(CCR6) (Clone 29-2L17) | Biolegend | Cat#129823; RRID: AB_2715923 |
| Brilliant Violet 785™ anti-mouse CD127(IL-7Ra) (Clone A7R34) | Biolegend | Cat#135037; RRID: AB_2565269 |
| Brilliant Violet 785™ anti-mouse CD25(Clone PC61) | Biolegend | Cat#102051; RRID: AB_2564131 |
| Brilliant Violet 785™ anti-mouse CD45.2(Clone 104) | Biolegend | Cat#109839; RRID: AB_2562604 |
| FITC anti-mouse CD3e (Clone 145-2C11) | Biolegend | Cat#100306; RRID: AB_312671 |
| FITC anti-mouse NK1.1(Clone PK136) | Biolegend | Cat#108706; RRID: AB_313392 |
| FITC anti-mouse TER119(Clone TER-119) | Biolegend | Cat#116206; RRID: AB_313707 |
| FITC anti-mouse CD5(Clone 53-7.3) | Biolegend | Cat#100606; RRID: AB_312735 |
| FITC anti-mouse CD19(Clone 6D5) | Biolegend | Cat#115506; RRID: AB_313640 |
| FITC anti-mouse Ly-6G/Ly-6C(Gr-1) (Clone RB6-8C5) | Biolegend | Cat#108406; RRID: AB_313370 |
| FITC anti-mouse/human CD11b (Clone M1/70) | Biolegend | Cat#101206; RRID: AB_312788 |
| FITC anti-mouse CD11c (Clone N418) | Biolegend | Cat#117306; RRID: AB_313775 |
| FITC anti-mouse CD45.2(Clone 104) | Biolegend | Cat#109806; RRID: AB_313442 |
| FITC anti-mouse CD45RB(B220) (Clone RA3-6B2) | Biolegend | Cat#103206; RRID: AB_312991 |
| BD Horizon™ BV650 anti-mouse T-bet (Clone 04-46) | BD Biosciences | Cat#564142; RRID: AB_2738616 |
| BD Pharmingen™ Alexa Fluor® 488 anti-mouse GATA3(Clone L50-823) | BD Biosciences | Cat#560077; RRID: AB_1645303 |
| BD Horizon™ PE-CF594 anti-mouse RORγt (Clone Q31-378) | BD Biosciences | Cat#562684; RRID: AB_2651150 |
| BD Pharmingen™ PE-Cy™7 anti-mouse GATA3(L50-823) | BD Biosciences | Cat#560405; RRID: AB_1645544 |
| BD Pharmingen™ Alexa Fluor® 647 anti-mouse T-bet (Clone O4-46) | BD Biosciences | Cat#561267; RRID: AB_10564093 |
| PE FOXP3 Monoclonal antibody (Clone FJK-16s) | eBioscience | Cat#12-5773-82; RRID: AB_465936 |
| PE-Cyanine7 FOXP3 Monoclonal antibody (Clone FJK-16s) | eBioscience | Cat#25-5773-82; RRID: AB_891552 |
| PE anti-mouse CD45.2 | Biolegend | Cat#109808; RRID: AB_313445 |
| Rat anti-mouse CD16/32 | Biolegend | Cat#101320; RRID: AB_1574973 |
| APC Rat anti-Integrinα3+β1 polyclonal antibody (Clone 976-1025/35-47) | BIOSS | Cat#bs-1057R; RRID: AB_10856085 |
| PE anti-mouse TCR γ/δ (Clone GL3) | Biolegend | Cat#118108; RRID: AB_313832 |
| APC-eFluor™ 780 anti-mouse CD45(Clone 30-F11) | eBioscience | Cat#47-0451-82; RRID: AB_1548781 |
| PE anti-mouse CD196 (CCR6) (Clone 29-2L17) | Biolegend | Cat#129804; RRID: AB_1279139 |
| PE-Cyanine7 anti-mouse IL-17A (Clone TC11-18H10.1) | Biolegend | Cat#506922; RRID: AB_2125010 |
| FITC anti-mouse CD3(Clone 17A2) | Biolegend | Cat#100203; RRID: AB_312660 |
| PE anti-mouse CD3(Clone 17A2) | Biolegend | Cat#100205; RRID: AB_312662 |
| APC anti-mouse T-bet (Clone 4B10) | eBioscience | Cat#17-5825-82; RRID: AB_2744712 |
| FITC anti-mouse/human KLRG1(Clone 2F1/KLRG1) | Biolegend | Cat#138410; RRID: AB_10643998 |
| APC-Cyanine7 anti-mouse/human KLRG1(Clone 2F1/KLRG1) | Biolegend | Cat#138425; RRID: AB_2566553 |
| PE anti-mouse CD218a(IL-18Ra) (Clone P3TUNYA) | eBioscience | Cat#12-5183-82; RRID: AB_2572617 |
| APC anti-mouse CD218a(IL-18Ra) (Clone A17071D) | Biolegend | Cat#157906; RRID: AB_2860735 |
| PE/Cyanine7 anti-mouse IL-33Rα (IL1RL1, ST2) (Clone DIH9) | Biolegend | Cat#145315; RRID: AB_2687366 |
| Biotin anti-mouse IL-33Rα (IL1RL1, ST2) (Clone DJ8) | MD Biosciences | Cat#101001B; RRID: AB_947551 |
| Alexa Fluor™ 700 anti-mouse CD103(Clone 2E7) | eBioscience | Cat#56-1031-82; RRID: AB_2637111 |
| Brilliant Violet 421™ anti-mouse/human IL-5(Clone TRFK5) | Biolegend | Cat#504311; RRID: AB_2563161 |
| eFluor™ 660 anti-mouse IL-13(Clone eBio13A) | eBioscience | Cat#50-7133-82; RRID: AB_2574279 |
| APC anti-mouse CD8b (Clone H35-17.2) | eBioscience | Cat#17-0083-81; RRID: AB_657760 |
| PE anti-mouse/human CD44(Clone IM7) | Biolegend | Cat#103008; RRID: AB_493687 |
| APC-Cyanine7 anti-mouse CD62L (Clone MEL-14) | Biolegend | Cat#104428; RRID: AB_830799 |
| APC anti-mouse CD133(Clone 315-2C11) | Biolegend | Cat#141207; RRID: AB_10898121 |
| BD Pharmingen™ Purified Mouse anti-GATA3(Clone L50-823) | BD Biosciences | Cat#558686; RRID: AB_2108590 |
| BD Horizon™ BUV737 Streptavidin | BD Biosciences | Cat#564293; RRID: AB_2869560 |
| Chemicals, peptides, and recombinant proteins | ||
| Recombinant Human IL-2 | Peprotech | Cat#200-2 |
| Recombinant Murine IL-7 | Peprotech | Cat#217-17 |
| DNase I | Roche | Cat#10104159001 |
| Collagenase P | Roche | Cat#11213865001 |
| Collagenase IV | Sigma-Aldrich | Cat#C5138 |
| Hyaluronidase | Harveybio | Cat#EZ2176 |
| LXY2 | Chinapeptides | N/A |
| Foxp3/Transcription Factor Staining Buffer Set | eBioscience | Cat#00-5523-00 |
| Paraformaldehyde | Sigma-Aldrich | Cat#P6148 |
| Tissue-Tek® O.C.T. Compound | Sakura | Cat#4583 |
| Neutral Protease (Dispase) | BioRuler | Cat#RH73624-25G |
| RPMI 1640 | Gibco | Cat#31800105 |
| Bovine serum albumin, fraction V | Sangon | Cat#A500023-0100 |
| Triton X-100 | VWR | Cat#0694-1L |
| Penicillin-Streptomycin | Gibco | Cat#15140-122 |
| 4-Hydroxytamoxifen, 99% | Macklin | Cat#A872541-5mg |
| Fetal Bovine Serum | Gibco | Cat#10099141C |
| Tween-20 | VWR | Cat#0777-1L |
| eBioscience™ Cell Stimulation Cocktail (500X) | Invitrogen | Cat#00-4970-93 |
| eBioscience™ Monensin Solution (1000X) | Invitrogen | Cat#00-4505-51 |
| DTT | BBI | Cat#A620058 |
| DAPI Stain Solution | BBI | Cat#E607303 |
| eBioscience™ Fixable Viability Dye eFluor™ 506 | Invitrogen | Cat#65-0866-14 |
| Critical commercial assays | ||
| Single Cell Full Length mRNA Amplification Kit | Vazyme | Cat#N712 |
| TruePrep DNA Library Prep Kit V2 for Illumina | Vazyme | Cat#TD501 |
| BD™ Mouse Immune Single-Cell Multiplexing Kit | BD Biosciences | Cat#633793 |
| Hyperactive pG-MNase CUT&RUN Assay Kit for Illumina | Vazyme | Cat#HD102 |
| Deposited data | ||
| Raw data of RNA-seq and ATAC-seq | This paper | GSA: CRA010739 |
| RNA-seq data of GATA3 sufficient and deficient ILC2 | Yagi et al.9 | GEO: GSE47851 |
| RNA-seq data of GATA3 sufficient and deficient ILC3 | Zhong et al.13 | GEO: GSE71198 |
| ATAC-seq data of canonical ILC2 and ILC3 | Shih et al.35 | GEO: GSE77695 |
| Experimental models: Organisms/strains | ||
| Mouse: Gata3 fl/fl | NIAID -Taconic repository | Line 355; PMID:15475959 |
| Mouse: Gata3 ZsG-fl/fl | Dr. JInfang Zhu, NIAID, NIH | PMID: 36466899 |
| Mouse: Gata3 fl/fl VavCre | NIAID -Taconic repository | Line 8446; PMID:15475959 |
| Mouse: Id2-YFP | Dr. Jonathan R. Keller, NCI, NIH | PMID: 25051963 |
| Mouse: Rorc-Cre | The Jackson Laboratory | Cat#JAX: 022791; RRID: IMSR_JAX:022791 |
| Mouse: Rosa26 TdTamato | The Jackson Laboratory | Cat#JAX: 007914; RRID: IMSR_JAX:007914 |
| Mouse: CD45.2 Rag2 −/−Il2rg −/− | The Jackson Laboratory | Cat#JAX: 014593; RRID: IMSR_JAX:014593 |
| Mouse: Rag2 −/− | The Jackson Laboratory | Cat#JAX: 008449; RRID: IMSR_JAX:008449 |
| Mouse: Il22 Cre | The Jackson Laboratory | Cat#JAX: 027524; RRID: IMSR_JAX:027524 |
| Mouse: Il5 iCre | The Jackson Laboratory | Cat#JAX: 030926; RRID: IMSR_JAX:030926 |
| Mouse: Rosa26 YFP | The Jackson Laboratory | Cat#JAX: 006148; RRID: IMSR_JAX:006148 |
| Mouse: Rorc fl/fl | The Jackson Laboratory | Cat#JAX: 008771; RRID: IMSR_JAX:008771 |
| Mouse: CreERT2 | The Jackson Laboratory | Cat#JAX: 007001; RRID: IMSR_JAX:007001 |
| Mouse: C57BL/6 | Department of Laboratory Animal Science, PKUHSC | N/A |
| Mouse: B6. CD45.1 | Department of Laboratory Animal Science, PKUHSC | N/A |
| Software and algorithms | ||
| FlowJo v10 | Tree Star | http://www.flowjo.com/ |
| GraphPad Prism 7 | GraphPad | http://www.graphpad.com/ |
| ImageJ | NIH | https://imagej.net/ |