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Primer sequence for the mutants.
| Specificity | Sequence (5′-3′) |
| pPLproCys102Ala | F: CATTAATCCAAGCGTTGTTGTCAGTC |
| R: CAACGCTTGGATTAATGCAATTTGTCTTG | |
| pPLproArg252Ala | F: GACCATTAGCATTAGAACCGCTATAAC |
| R: CTAATGCTAATGGTCATTACACCTATTACG | |
| pPLproHis255Ala | F: GTGTAAGCACCATTCCTATTAGAACCG |
| R: GAATGGTGCTTACACCTATTACGATAACC |
