Figure 3.

Effect of RFP on PRRSV life cycle. (A) PRRSV was incubated with RFP at the indicated concentration for 0.5, 1 or 2 h at 37°C, and then the virus were used to infect Marc-145 cells. At 24 hpi, The N and Nsp4 proteins of PRRSV were measured by western blot. (B) Schematic of the experimental approach of adding the drug at different time points. Marc-145 cells were infected with GDBY1, followed by addition of RFP at designated times. Red bars represent the PRRSV infection period, green bars represent RFP treatment. (C–F) Viral binding, internalization, replication, and release were performed as described in Material and Methods.