FIGURE 5.
The effect of paracetamol on spontaneous phasic contractions, cch-induced contractions, and excitatory electrical field stimulation. (Top) Histograms show the effect of paracetamol (10–5 M to 3 × 10−3 M) plus HBB (10–7 to 10–5 M) on spontaneous phasic contractions (A), cch-induced contractions (B), and excitatory electrical field stimulation (C). Dunnett’s post hoc test was done after ANOVA. # p < 0.05, #### p < 0.0001 compared with basal conditions and *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared with paracetamol 3 mM (A–C). (Bottom) Histograms show the effect of paracetamol (100 µM) plus HBB (10–7 to 10–5 M) compared with HBB (10–7 to 10–5 M) plus vehicle on spontaneous phasic contractions (D), cch-induced contractions (E), and excitatory electrical field stimulation (F). Two-way ANOVA (concentration x treatment) was performed to test a potential complementary effect of paracetamol and HBB. Notice that although a concentration dependent reduction was observed in both groups (concentration: p < 0.0001) the combination of paracetamol and HBB was not different from the effect of HBB plus vehicle (treatment p > 0.05). Data were normalized (i.e., 100%) to the basal AUC or the electrical field stimulation amplitude before drug addition. Data were expressed as mean ± SEM.