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Detection of the unique flanking region of HTLV-1 provirus in a series of FPM1 cells. Genomic DNA (0.5 μg) was obtained from rat HTLV-1-infected cell lines and was subjected to PCR amplification with primer pair px1 and px4 (px) or U5-4 and Gen1 (the integration site). The amplified products were separated on 2% agarose gel and stained with ethidium bromide.
