Skip to main content
. 1999 Jul;73(7):6048–6055. doi: 10.1128/jvi.73.7.6048-6055.1999

FIG. 1.

FIG. 1

Schematic representation of the adenovirus type 5 E4 region and construction of plasmids for generating the Av4orf3nBg vector. (A) ORFs of the E4 region are indicated by open boxes. ORF3 is represented by a hatched bar. (B) Construction of pAv4orf3nBg. pdl23 is a plasmid that contains the 3′ end of the adenovirus type 5 genome (bp 16746 to 35936) with deletions in the E2a and E3 regions (17). A PacI-XbaI fragment from pdl23 was replaced with a PacI-XbaI fragment containing the E4 promoter and the ORF3 gene (bp 34936 to 34330), generating pdl234. The NotI-SpeI fragment from pdl234 was replaced with a NotI-SpeI fragment derived from pAvS6a (35) to generate pdl234LE, which contains the left-end ITR, Ψ, RSV promoter, TPL, regions with E3 and E4 deleted, and right ITR. The single infectious plasmid, pAv4orf3nBg, was generated by inserting an SpeI fragment of 25,633 bp flanking the nucleus-localizing β-galactosidase reporter transgene (nBg) gene and the region with E2a deleted. E4p, E4 promoter; RSV, heterologous RSV promoter.