FIG. 6.

Quantitative evaluation of DNA delivery to the nucleus at various times after a 10-min infection. A549 cells were infected with Ad5 or Ad5f7 (10³ particles/cell) at 37°C for 10 min. The cells were washed and then incubated for 0 to 8 h at 37°C. The cells were then harvested, and DNA was extracted from the total cells or isolated nuclei. Ad DNA and A549 DNA were quantified by TaqMan PCR using probes for the Ad E2 region and for the cellular β-actin gene. Data are percentages of Ad DNA delivered to the nucleus, i.e., a ratio of Ad genome copies to β-actin gene copies from the nuclear fraction versus those from the total cell lysate. Shown are DNA analyses of Ad5 (○) and Ad5f7 (●). Data are mean values and standard errors from three experiments for each vector.