FIG. 7.

Comparison of localization of the Cy3-Ad capsid and Ad DNA localization (FISH) for Ad5 and Ad5f7 at various times after infection. The Ad capsids (upper panels) were detected by Cy3 fluorescence, and Ad DNA (lower panel) was detected with FITC–anti-DIG after hybridization with DIG-Ad DNA probe. Micrographs show an individual nucleus with adjacent cytoplasm. Arrows indicate colocalization of Ad capsid and DNA. Ad5 DNA was dissociated from its capsid within 4 h following infection, while Ad5f7 DNA was still colocalized with its capsid at 4 h. Bar = 5 μm.