Figure 6.

Effect of Saroglitazar (SARO), Capparis spinosa (CS), and Quercetin (QC) on the mRNA expression of α-SMA, Collagen1α (Col-1α), Collagen IV (Col-IV), and Transforming growth factor beta (TGF-β1) in activated HSCs

The relative expression of TGF-β1 (A), Col-1α (B), Col-IV (C), and α-SMA (D) genes in HSCs activated with High-fat diet (HFD) increased significantly, and after treatment with SARO, QC, CS, and QC+CS, it was significantly reduced. The relative chain‐fold (2^−ΔΔCt) of each gene was indicated as a column. **P<0.01, ***P<0.001, and ****P<0.0001 vs control group, and ^#P<0.05, ^##P<0.01, and ^###P<0.001 vs HFD group. (E) Histopathological evaluation of the NASH model after treatment with SARO, QC, CS, and QC+CS. Images of liver tissue stained with Masson Trichrome at 100X magnification from different treatment groups. (F: Fibrosis)