Table 2.
Primer pairs sequences
| Gene | Forward primer | Reversed primer |
|---|---|---|
| GAPDH | CTCTCTGCTCCTCCCTGTTC | CGATACGGCCAAATCCGTTC |
| SREBP-1c | TCTTGACCGACATCGAGACAT | CCTGTGTCTCCTGTCTCACC |
| FAS | CCCGGACCCAGAATACCAAG | TCTTCAAGTCACACGAGGTG |
| ACC | TTAAGGGGTGAAGAGGGTGC | CACTTCCAAAGACCTAGCC |
| PPARγ | CGAGTGTGACGACAAGGTGA | ACGCTTCTTCAATCTGTCTG |
| PPARα | TGGTGCATTTGGGCGTATCT | CACGAGCGCTAAGCTGTGA |
| CPT-1α | AGCCCTGAGACAGACTCACA | ATCACGAGGGTCCGTTTTCC |
| IL-1β | TGCCACCTTTGACAGTGATG | TGATGTGCTGCTGCGAGATT |
| IL-6 | CCAGTTGCCTTCTTGGGACT | TGCCATTGCACAACTCTTTC |
| TNF-α | ATGGGCTCCCTCTCATCAGT | GCTTGGTGGTTTGCTACGAC |
| MCP-1 | TGAGCACGTTTCAGTGAGCA | CCAACCTGCTTTACACCTAA |
| NOX1 | AGGCTCCAGACCTCCATTGA | AAGGCAAGGCAGTTCCGAG |
| NOX2 | GGCATTCGTAGTACAGCTCA | ATTGGTCCTCGGGAGTCAGA |
| NOX4 | TGGCCAACGAAGGGGTTAAA | ACACAATCCTAGGCCAACA |
| TGF-β1 | CTGCTGACCCCCACTGATAC | GGGGCTGATCCCGTTGATT |
| COL-1α | CCGATGGATTCCAGTTCGAGT | GGGACTTCTGAGGTTGCCA |
| COL-IV | GGCCCTTCATTAGCAGGTGT | GCTGGTGTGCATCACAAGG |
| α-SMA | GCCATCTTCATTGGGATGGA | CCCTGACAGGACGTTGTTA |
The amount of each gene was normalized to the amount of glyceraldehyde 3-phosphate dehydrogenase (GAPDH)