Fig. 1.

BET activity is required for neural crest formation. (A) In situ hybridization examining the expression of neural crest factors snai2 and foxd3 following treatment with vehicle or inhibitor (250 µM IBET, 10 µM JQ1, 100 µM AZD5153). Embryos were collected at stage 15 (mid neurula). (B) In situ hybridization examining expression of neural crest factors sox9 and snai1, epidermal marker krt12.4, mesodermal marker myoD1, placodal marker six1 and neural plate marker sox3 following treatment with vehicle or IBET. Embryos were treated at two-cell stage and collected at stage 15 (mid neurula). (C) In situ hybridization examining expression of snai2 and foxd3 in Wnt/Chrd-induced explants treated with vehicle or IBET. Explants were collected at stage 18 (late neurula). (D) In situ hybridization examining expression of neural crest factors snai2 and foxd3 following treatment with vehicle or IBET at the indicated stage (two-cell, 6.5 or 9). Embryos were collected at stages 13, 15 or 17 (early, mid or late neurula, respectively). Scale bars: 250 μm.