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. 2024 Jul 10;15:1434272. doi: 10.3389/fimmu.2024.1434272

Figure 3.

Figure 3

Effects of Akt and PKA inhibition on ACLY phosphorylation, LPS-induced pro-inflammatory cytokine production and basal histone acetylation in THP-1 cells. (A) Western blot analysis of ACLY phosphorylation in ACLY WT cells upon treatment with 10 µM MK2206 or 10 µM H-89 for 30 minutes. (B) Western blot analysis of PRAS40 phosphorylation in ACLY WT cells upon treatment with 10 µM MK2206 or 10 µM H-89 for 30 minutes. (C) Western blot analysis of CREB phosphorylation upon treatment with 10 µM H-89 or 50 µM forskolin for 30 minutes (D) mRNA expression of IL6, CCL2 and CCL4 in ACLY WT and S455A THP-1 cells following treatment with 10 µM MK2206 or 10 µM H-89 for 30 minutes and 100 ng/ml LPS for 3 hours. (E) Western blot analysis of histone H3 acetylation at K9, K27, K14 and K23 in ACLY WT and S455A cells after treatment with 10 µM MK2206 or 10 µM H-89 for 30 minutes. Data represent mean values ± SE of 3-7 independent experiments. * p<0.05, ** p<0.01, *** p<0.001.