Figure 2.

Influence of maternal 1‐NP exposure on autism‐like behavior in weaning offspring. 30 pregnant mice orally received different dose of 1‐NP (0, 10, 100 µg kg⁻¹) daily from GD0 to GD17. All pregnant mice gave birth naturally. A–F) Three‐chamber social interaction test was used to evaluate autism‐like behaviors in weaning offspring (PND28). A) Pattern diagram of social behavior in weaning offspring. B) Sniffing time spent in male weaning offspring. C) Sniffing time spent in female weaning offspring. D) Pattern diagram of social novelty behavior in weaning offspring. E) Sniffing time spent in male weaning offspring. F) Sniffing time spent in female weaning offspring. G–L) Three‐chamber social interaction test was used to evaluate autism‐like behaviors in adult offspring (PND70). G) Pattern diagram of social behavior in adult offspring. H) Sniffing time spent in male adult offspring. I) Sniffing time spent in female adult offspring. J) Pattern diagram of social novelty behavior in adult offspring. K) Sniffing time spent in male adult offspring. L) Sniffing time spent in female adult offspring. N = 9–11. S1, stranger 1. S2, stranger 2. E, empty cage. *P < 0.05.