Figure 5.

Influence of gestational 1‐NP exposure on migration of interneurons in fetal brain. A–H) 10 pregnant mice orally received different dose of 1‐NP (0, 100 µg kg⁻¹) daily from GD0‐GD17. Fetal mPFC was collected on GD18. A,B) GAD67 protein in male fetuses was detected by Western blotting. C) GAD67⁺ interneurons in male fetuses were determined using IF. D) The distribution of GAD67⁺ interneurons was analyzed in different zones (MZ, CP, IZ, SVZ/VZ) of the mPFC. N = 4. Original magnification: 400×. *P < 0.05. E,F) The expression of GAD67 in female fetuses was measured using Western blotting. G) GAD67⁺ interneurons in female fetuses were determined using IF. H) The distribution of GAD67⁺ interneurons was analyzed in different zones (MZ, CP, IZ, SVZ/VZ) of mPFC. N = 4. Original magnification: 400×. *P < 0.05. **P < 0.01. I–X) 10 pregnant mice orally received different dose of 1‐NP (0, 100 µg kg⁻¹) daily from GD0‐GD13. Fetal forebrains were collected on GD14. I,J) GAD67 in fetal forebrain was measured by Western blotting. K) GAD67 interneurons in forebrain cortex were determined using IF. L) GAD67 interneurons were analyzed in 1–3 areas of forebrain cortex. N = 4. Original magnification: 400×. *P < 0.05. M–X) Interneuron migration‐related genes in fetal forebrain were measured using RT‐PCR. M) Cxcl12. N) Cxcr4. O) Cxcr7. P) Slit1. Q) Efna5. R) Arx. S) Nrp1. T) Nrp2. U) Sema3A. V) Nrg1. W) Erbb4. X) Sema3F. N = 5. *P < 0.05. MZ, marginal zone; CP, cortical plate; IZ, intermediate zone; SVZ, subventricular zone; VZ, ventricular zone.