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. 2024 May 16;11(28):2306294. doi: 10.1002/advs.202306294

Figure 8.

Figure 8

Effects of supplementation with α‐KG on 1‐NP‐evoked hypohydroxymethylation of interneuron migration‐related genes and interneuron migration inhibition in fetal forebrain. 20 pregnant mice orally received different dose of 1‐NP (0, 100 µg kg−1) daily from GD0‐GD13. Fetal forebrains were collected on GD14. A–F) The content of 5hmC at 5hmC‐rich CpG sites of interneuron migration‐related genes was measured in fetal forebrain. A) The content of 5hmC in the site (chr1: 32009246) of Nrg1 gene. B,C) The content of 5hmC in two CpG sites (chr8: 69107743 and 69107866) of Erbb4 gene. D‐F) The content of 5hmC in three CpG sites (chr9: 107709269, 107709348 and 107710238) of Sema3F gene. N = 5. **P < 0.01. G–I) The mRNAs of interneuron migration‐related genes in fetal forebrain were measured using RT‐PCR. G) Nrg1. H) Erbb4. I) Sema3F. N = 5. *P < 0.05. **P < 0.01. J,K) The number of GAD67 interneurons was measured in 1–3 areas of forebrain cortex. N = 4. **P < 0.01.