Figure 9.

Effects of gestational α‐KG supplementation on interneurons in weaning offspring. 20 pregnant mice orally received different dose of 1‐NP (0, 100 µg kg⁻¹) daily from GD0‐GD17. All pregnant mice gave birth naturally. A‐E) Weaning male offspring was sacrificed on PND28 and the mPFC was harvested. A,B) GAD67 was measured by Western blotting. C–E) GAD67⁺ and NeuN⁺ neurons in mPFC were measured by IF. C) Representative photograph. D) The proportion of GAD67⁺ to NeuN⁺ neurons in each subfield of the mPFC. E) Percentage of NeuN⁺ neurons in the mPFC. N = 4. Original magnification: 400×. *P < 0.05. **P < 0.01. F–J) Weaning female offspring was sacrificed on PND28 and the mPFC was collected. F,G) GAD67 was measured by Western blotting. H–J) GAD67⁺ and NeuN⁺ neurons in the mPFC were measured using IF. H) Representative photograph. I) The proportion of GAD67⁺ to NeuN⁺ neurons in each subfield of the mPFC. J) Percentage of NeuN⁺ neurons in the mPFC. N = 4. Original magnification: 400×. *P < 0.05. **P < 0.01.