Figure 10.

Effects of gestational α‐KG supplementation on 1‐NP‐induced mIPSC transmission disorder and autism‐like behaviors. 20 pregnant mice orally received different dose of 1‐NP (0, 100 µg kg⁻¹) daily from GD0 to GD17. All pregnant mice gave birth naturally. A–C) mIPSC in the mPFC was measured in male offspring. A) Representative photograph. B) Frequency of mIPSC. C) Amplitude of mIPSC. N = 13 excitatory neurons from three mice. **P < 0.01. D–F) mIPSC in the mPFC was measured in female offspring. D) Representative photograph. E) Frequency of mIPSC. F) Amplitude of mIPSC. N = 13 excitatory neurons from three mice. **P < 0.01. G–J) Three‐chamber social test was used to evaluate autism‐like behavior in offspring. G) Sniffing time spent in sociability test in male offspring. H) Sniffing time spent in social novelty test in male offspring. I) Sniffing time spent in sociability test in female offspring. J) Sniffing time spent in social novelty test in female offspring. N = 9‐11. *P < 0.05.