Fig. 6.

The broad-spectrum MMP inhibitor Marimastat rescues vascular ballooning in constitutively active c-Src in 3D bead sprouting. (A) Representative images of HUVECs transduced with c-Src WT, mutants (CA or DN) or control (Ctrl; empty vector mScarlet) (magenta) grown in a 5 mg/ml fibrin gel bead sprouting assay for 7 days and treated every 2 days with DMSO vehicle control or 5 µM Marimastat before fixing. Immunofluorescence staining was performed for F-actin (phalloidin; green) and nuclei (DAPI; blue). Quantification of sprout parameters of vascular area (B), number of sprouts per bead (C), and shape of vascular area (D). n=3 independent experiments. All data are represented as mean±s.e.m. with individual data points indicated and colours representing independent experiments. **P<0.01, ***P<0.001, ****P<0.0001 (two-way ANOVA with Sidak's multiple comparisons test).