Figure 3.

OP-B inhibits the invasion and migration of A549 cells in vitro and in vivo. (A) Transwell migration and invasion assays were performed to examine cell migration and invasion in A549 cells. Representative images of migrated or invaded cells are displayed (magnification, ×200). (B) Columns indicate the mean ± SD of triplicate experiments (***P<0.001, independent Student's t-test). (C) Wound healing assays were used to investigate the motility of A549 cells treated with OP-B, and representative images are shown (magnification, ×40). (D and E) A549 cells were treated with or without 10 µmol/l OP-B for 24 h, and then, the expression levels of vimentin, N-cadherin, E-cadherin, ZO-1, Snail, Slug, TCF8/ZEB1, p-SRC (Tyr416), p-FAK (Tyr397), p-Stat3 (Tyr705), Stat3, p-Stat5 (Tyr694) and Stat5 were detected by western blotting. β-actin was used as a loading control. The experiment was repeated three times and yielded similar results. OP-B, Ophiopogonin B. OP-B inhibits the invasion and migration of A549 cells in vitro and in vivo. (F) OP-B inhibits the lung metastasis of A549 cells in vivo. Representative images of H&E-stained metastatic lung nodules. OP-B, Ophiopogonin B.